Number of invA mRNA copies in late exponential phase cells

Value 1 copies/CFU
Organism Bacteria Salmonella enterica
Reference González-Escalona N, Hammack TS, Russell M, Jacobson AP, De Jesús AJ, Brown EW, Lampel KA. Detection of live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse transcriptase real-time PCR targeting invA mRNA. Appl Environ Microbiol. 2009 Jun75(11):3714-20PubMed ID19376910
Method DNA detection methods used for analysis of foods, are subject to the major concern of false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assay with an RNA internal control was developed.
Entered by Uri M
ID 104213