Experimental in vitro Constant for binding of repressor to O3 locus of lac operator

Range 0.016-1 nM
Organism Bacteria Escherichia coli
Reference Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Kuhlman T, Phillips R. Transcriptional regulation by the numbers: applications. Curr Opin Genet Dev. 2005 Apr15(2):125-35PubMed ID15797195
Primary Source Lin S, Riggs AD. The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes. Cell. 1975 Feb4(2):107-11.PubMed ID1092468
Method By equilibrium competition experiments, the dissociation constant (K(RD)) of lac repressor for E. coli DNA carrying a deletion of the lac operon was measured at a variety of salt concentrations.
Entered by Uri M
ID 103600