Number of mitochondrial DNA per mitochondrion

Value 2.6 copies/mitochondrion Range: ±0.3 copies/mitochondrion
Organism Mammals
Reference Robin ED, Wong R. Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells. J Cell Physiol. 1988 Sep136(3):507-13. abstractPubMed ID3170646
Method P.509 left column 3rd paragraph: "Mitochondria were isolated from cell suspensions according to the "no gradient" method of Bogenhagen and Clayton (Bogenhagen and Clayton, 1974)." P.509 left column bottom paragraph: "Quantitation of mtDNA: Mitochondrial DNA (mt DNA) was measured using the method of Tapper and Clayton (Murphy et al., 1984)." P.509 right column 2nd paragraph: "Mitochondrial counting: Isolated mitochondria from all cell types were stained with Rhodamine 123 according to the method of Johnson, Walsh, and Chen (Johnson et al., 1980)." P.509 right column 3rd paragraph: "Enzyme assays: Cytochrome aa3 was measured spectrophotometrically by the method of Simon et al. (1973)." P.509 right column 4th paragraph: "Malate dehydrogenase: Malate dehydrogenase was measured spectrophotometrically by the method of Rudolph, Baugher, and Beissner (Rudolph et al., 1977)."
Comments P.511 left column 2nd paragraph: "MtDNA/mt (or per mitochondrial fragment) shows almost no variation between values in any single cell type,between different cell types, and between cells from various mammalian species." Abstract: "Mt DNA/mitochondrion was essentially constant in all cell types (mean 2.6±0.30 SE mitochondrial DNA molecules/mt)."
Entered by Uri M
ID 102918