Number of nuclear pores per nucleus

Value 119 pores/nucleus
Organism Budding yeast Saccharomyces cerevisiae
Reference Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. J Cell Biol. 1977 Jun73(3):748-60. p. 752 table 1PubMed ID406262
Primary Source [17] Hartwell LH. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol. 1970 Dec104(3):1280-5.PubMed ID16559104
Method P.749 right column top paragraph: "Yeast (Saccharomyces cerevisiae) was grown according to Hartwell (primary source) at 24°C or with the same medium but containing 20% glycerol. After several days, the yeast adapted to this condition but grew very slowly. Determinations of nuclear diameter by phase-contrast microscopy, of height by electron microscopy, and of pore frequency by freeze-etching were done on cells during the exponential growth phase (48 h after plating)." P.753 right column 3rd paragraph: "[Investigators] decided to extend the number of different cell lines and select them according to different DNA content. Table I provides the summary of the data collected during this extended investigation, and reveals an obvious increase in nuclear surface with increasing DNA content: this increase is not proportional, however."
Comments P.751 right column 2nd paragraph: "The numbers in Table I represent the corrected values. Briefly, the indentation at the cytocenter results in a nuclear shape similar to the shape of an indented ball. Since most cells will appear round when the indentation is parallel to the optical axis, the volume will be extremely overestimated if the formula for a sphere is used."
Entered by Ben Marks
ID 101395