Leaf area (leaves 1, 2 & 6)

Range 30mm^2 leaf 1: 32mm^2 leaf 2: 205mm^2 leaf 6 figure - link mm^2
Organism Thale cress Arabidopsis thaliana
Reference Gonzalez et al., Increased leaf size: different means to an end. Plant Physiol. 2010 Jul153(3):1261-79. doi: 10.1104/pp.110.156018. p.1267 fig. 4APubMed ID20460583
Method p.1263 left column top paragraph:"Leaf areas were measured at individual leaf positions of plants harvested 22 d after stratification (DAS), which is just before bolting occurs in both systems. The growth measurements were performed at this time point to decrease the potential influence of the flowering time on leaf number and/or size." "For the leaf area analysis, leaves were harvested daily from eight to 10 plants grown in vitro (or in soil). The leaves were cleared with 100% ethanol, mounted in lactic acid on microscope slides, and photographed. The leaf area was determined with the ImageJ software ( link )." Extracted visually from figure 4A
Comments p.1262 right column 4th paragraph:"Final organ size is the result of the action of two processes, cell division and cell expansion. To analyze the extent to which cell proliferation and/or cell expansion contribute to enlarged leaves, the leaf epidermal cell number and size were analyzed in the five IYG [intrinsic yield gene] lines. The measurements were carried out at 22 DAS for BRI1 and 30 DAS for GRF5, JAW, AVP1, and GA20OX1. The cell number in all transgenic lines was higher than that of the wild-type controls (Fig. 4B). The increase was particularly high in GRF5 and JAW (more than 2-fold)."
Entered by Uri M
ID 107043