Dimensions and shapes of E. coli K-12 at various conditions

Range Table - link
Organism Bacteria Escherichia coli
Reference Zaritsky A, Woldringh CL. Chromosome replication rate and cell shape in Escherichia coli: lack of coupling. J Bacteriol. 1978 Aug135(2):581-7 p.583 table 1PubMed ID355235
Method Coulter counter or Electron microscopy. Grown on glucose and casaminoacids. Abstract: "The dimensions of Rep- cells of Escherichia coli K-12 were measured and compared with those of their Rep+ isogenic cells (both Thy-), Rep- cells cultivated identically were longer (but not wider), even though both strains were wider when the rate of chromosome replication was slowed down by lowering the thymine concentration supplied."
Comments P.582 right column bottom paragraph: "Shape changes with doubling time: The study of cell dimensions at different steady-state growth conditions, which has proved fruitful before (refs 7, 10, 17-20, 32, 33, 35, 40), was exploited again, and the same strains were cultivated in glucose-Casamino Acids-supplemented Medium to obtain shorter τ. Steady-state conditions could be reached only when deoxyguanosine was added (ref 38), and the cells looked as shown in Fig.3. Further experiments are summarized in Table 1. The most striking observation was the decrease in the average length of the Rep- cell compared to that in glucose minimal medium." Table gives diameter and length of E. coli cells for different C period (time to replicate chromosome) and generation time. Cell diameter range 0.68-1.04 µm. Length range 2.62-4.00 µm. See BNID 100002,103714,104825,104333, 102065,100001
Entered by Uri M
ID 104113