Genome size measured by flow-cytometry

Value 1.57e+8 base pairs
Organism Thale cress Arabidopsis thaliana
Reference Bennett MD, Leitch IJ, Price HJ, Johnston JS. Comparisons with Caenorhabditis (approximately 100 Mb) and Drosophila (approximately 175 Mb) using flow cytometry show genome size in Arabidopsis to be approximately 157 Mb and thus approximately 25% larger than the Arabidopsis genome initiative estimate of approximately 125 Mb. Ann Bot (Lond). 2003 Apr91(5):547-57. p.550 left column 4th paragraphPubMed ID12646499
Method Flow cytometry. P.550 left column 4th paragraph: "When C. elegans and arabidopsis were co-run in mixtures, the 2C peak for arabidopsis averaged approx. 57 % above that for C. elegans (Fig. 1D), giving a 1C DNA amount of approx. 157 Mb for arabidopsis based on an estimate of 100 Mb for C. elegans."
Comments P.554 right column bottom paragraph: "Complete sequencing of the arabidopsis genome until no gaps remain at telomeres, nucleolar organizing regions [NORs] or centromeres is still needed to provide the first precise angiosperm C-value as a benchmark calibration standard for plant genomes, and to ensure that no genes have been missed in arabidopsis, especially in centromeric regions, which are clearly larger than once imagined." See BNID 105472 for original genome size estimate of 125Mb.
Entered by Uri M
ID 104000