Spread surface area of wildtype bovine aortic endothelial (BAE) cell

Value 1450 µm^2 Range: ILK silenced cells: 3,000 expressing GFP : 6,300 expressing Rac-V1 µm^2
Organism Mammalian tissue culture cell
Reference Boulter E, Grall D, Cagnol S, Van Obberghen-Schilling E. Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase. FASEB J. 2006 Jul20(9):1489-91 p.E646 fig 4D and p.E647 fig.5BPubMed ID16723384
Method Morphometric analysis. P.E646 caption to figure 4B: "Cells were plated on fibronectin-coated coverslips 1 h before fixation and staining with Alexa-phalloidin to label F-actin."
Comments The cells whose surface is given in 'Range' [both those expressing GFP and those expressing Rac-V1] were ILK (integrin linked kinase)-silenced. P.E646 caption to figure 4B: "Morphometric analysis revealed a 1.58-fold increase (P<0.001) in mean surface area of ILK-GFP-F expressing BAE cells, relative to control (GFP) cells" Values extracted visually from figures.
Entered by Uri M
ID 105878