Comparison of protein abundance between S. pombe and S. cerevisiae
| Range | Table - link |
|---|---|
| Organism | Yeast |
| Reference | Wu JQ, Pollard TD. Counting cytokinesis proteins globally and locally in fission yeast. Science. 2005 Oct 14 310(5746) Supporting Online Material pp.16-18 table S2PubMed ID16224022 |
| Primary Source | [S22] Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS. Global analysis of protein expression in yeast. Nature. 2003 Oct 16 425(6959):737-41. [S23] Kim K, Yamashita A, Wear MA, Maéda Y, Cooper JA. Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. J Cell Biol. 2004 Feb 16 164(4):567-80. DOI: 10.1083/jcb.200308061 [S24] Sherman F. Getting started with yeast. Methods Enzymol. 2002 350: 3-41.PubMed ID14562106, 14769858, 12073320 |
| Method | Abstract: "[Investigators] used fluorescence microscopy to measure global and local concentrations of 28 cytoskeletal and signaling proteins fused to yellow fluorescent protein (YFP) in the fission yeast Schizosaccharomyces pombe. Native promoters controlled the expression of these functional YFP fusion proteins. Fluorescence measured by microscopy or flow cytometry was directly proportional to protein concentration measured by quantitative immunoblotting." |
| Comments | P.312 middle column 2nd paragraph: "Global cytoplasmic concentrations of cytokinesis proteins (Table 1) range from 0.04 μM formin Cdc12p (600 molecules per cell) to 63.2 μM actin (1.43 million molecules per cell). The concentration of myosin-II light chain Cdc4p (4.8 μM) is 10-fold higher than that of Myo2p (0.45 μM), which is in keeping with evidence for functions beyond its partnership with myosins (refs 11–14). Most of the proteins studied are more abundant in S. pombe than in haploid strains of S. cerevisiae (table S2), in part because of the 1.7-fold higher protein content per cell of S. pombe (See the fission yeast handbook (available at www.sanger.ac.uk/PostGenomics/S_pombe/docs/nurse_lab_manual.pdf ) & primary source S[24])." |
| Entered by | Uri M |
| ID | 102303 |