Cyanopodovirus burst size in Synechococcus WH7803

Value 81 Virions/burst Range: Table - link Virions/burst
Organism Cyanophage P60 (Synechococcus WH7803)
Reference Brown, C.M., Lawrence, J.E., and Campbell, D.A. (2006) Are phytoplankton population density maxima predictable through analysis of host and viral genomic DNA content? J Mar Biol Assossiation UK 86: 491–498. DOI: link p.492 table 1
Primary Source Garza DR, Suttle CA. The Effect of Cyanophages on the Mortality of Synechococcus spp. and Selection for UV Resistant Viral Communities. Microb Ecol. 1998 Nov 36(3):281-292PubMed ID9852508
Method p.493 left column 2nd paragraph [general burst size measurement method]:"Burst sizes were derived from either indirect estimates from dilution to- extinction assays of infectivity (Suttle, 1993) or direct counts done with flow cytometry or transmission electron microscopy (TEM). Whenever possible, [investigators] used direct count estimates, since viral burst size estimates based on infectivity can underestimate viral particle numbers (Van Etten, 1983 Cotrell & Suttle, 1995)." p.493 right column 6th paragraph [Specifically for Synechococcus WH7803/P60 phage]:"Burst data for this podovirus were not available. As a proxy, researchers used a single estimate for viruses infecting Synechococcus in the Gulf of Mexico (primary source)."
Comments p.495 left column 4th paragraph:"For the cyanophages Cyanomyoviridae S-PM2 and Cyanopodoviridae P60, which both infect Synechococcus WH7803, [investigators] initially predicted burst sizes on the basis of one genome copy per host cell. The predicted burst of 12 for S-PM2 was lower than the 22 to 45 observed in nutrient-replete media (Wilson et al., 1996).The predicted burst of 50 for P60 was also lower than the 81 cited for bacteriophage in marine environments (primary source). A probable source of this discrepancy is the multiple genome copies found in cyanobacteria, with Synechococcus WH7803 typically carrying between one and three copies (Binder & Chisholm, 1995)."
Entered by Uri M
ID 104842