Number of Z-domain of protein A and streptavidin

Range Z-domain 280,000: streptavidin 160,000 molecules/cell
Organism Bacteria Escherichia coli
Reference Park M et al., Orientation and density control of proteins on solid matters by outer membrane coating: Analytical and diagnostic applications. J Pharm Biomed Anal. 2017 Jul 31. pii: S0731-7085(17)31544-3. doi: 10.1016/j.jpba.2017.07.043 p.3 right column top paragraphPubMed ID28797956
Primary Source [59] J. Jose, J.W. Chung, B.J. Jeon, R.M. Maas, C.H. Nam, J.C. Pyun Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor Biosens. Bioelectron., 24 (5) (2009), pp. 1324-1329 doi: 10.1016/j.bios.2008.07.067 [60] M. Park, J. Jose, S. Thommes, J.I. Kim, M.J. Kang, J.C. Pyun, Autodisplay ofstreptavidin, Enzyme Microb. Technol. 48 (4-5) (2011) 307–311 doi: 10.1016/j.enzmictec.2010.12.006PubMed ID19036573, 22112942
Comments P.3 left column bottom paragraph: "By densitometry analysis, the intensity of the band representing the expressed Z protein after SDS-PAGE was compared with the band intensity of OmpA, a natural occurring OM [outer membrane] protein with a constant number of 10^5 molecules per cell [primary source 59, ref 64]. By comparing the band intensities and putting them into relation with the corresponding molecular weights of the to proteins, the number of autodisplayed Z-domain and streptavidin was determined to be 2.8 × 10^5 and 1.6 × 10^5 molecules per cell, respectively [primary sources]. Hence, density of a protein displayed at the cell surface e.g. the Z-domain or streptavidin can be controlled by the promotor used for expression of the recombinant protein by autodisplay [ref 22]."
Entered by Uri M
ID 114158