| Comments |
P.7 bottom paragraph: "The correlation between genome and nuclear size has been known for over a century (Gregory, 2001, 2011, Umen, 2005). It is therefore tempting to speculate that nuclear size is determined by the amount of nuclear DNA. However, abundant phenomenological and experimental evidence demonstrates that other factors must contribute to nuclear size. Different cell types within the same species exhibit nuclear size differences, despite having the same genome content, and nuclear size varies during early development while the DNA amount per cell remains constant (Altman and Katz, 1976, Butler et al., 2009, Faro-Trindade and Cook, 2006, Oh et al., 2005, Thomson et al., 1998). Experimental manipulation of DNA content often has a minimal impact on nuclear size. In fission yeast, a 16-fold increase in nuclear DNA amount did not affect nuclear size (primary source) (Fig. 1)." |