| Comments |
P.210 right column 2nd paragraph: "While the endogenous 2μ plasmid is lost in less than 0.1‰ of the cell divisions (primary source), YEps [Yeast episomal plasmids] have a higher plasmid loss frequency. YEps that are created by the insertion of heterologous sequences into the complete 2μ plasmid are typically lost in about 1% of the cell divisions. YEps containing only the 2μ ORI and STB [a cis-acting sequence] sequences have plasmid loss frequencies of 1–5% in cir+ cells and of up to 50% in cir0 cells if REP1 and REP2 expression in trans is absent (Christianson et al., 1992, Futcher and Cox, 1984). The increased plasmid loss frequency of YEps can be attributed to decreased replication or amplification propensities and burdening overexpression of marker genes and other heterologous genes (Rose and Broach, 1990, Cakar et al., 1999, Karim et al., 2013)." |