||Fruit fly Drosophila melanogaster
||Kuromi, H., & Kidokoro, Y. (2003). Two synaptic vesicle pools, vesicle recruitment and replenishment of
pools at the Drosophila neuromuscular junction. Journal of neurocytology, 32(5-8), 551-565. p.558 right column top paragraphPubMed ID15034253
||P.558 left column 4th paragraph: "Sizes of SV pools: Because of continuing recycling of SVs, it is difficult
to measure the absolute size of pools. To circumvent this problem [investigators] used shibire, which is defective in endocytosis at non-permissive temperatures but behaves normally at room temperature (Poodry & Edgar, 1979 Kosaka & Ikeda, 1983 Koenig et al., 1983, 1989, 1993). This mutant is suited for determination of sizes of SV pools. After tetanic stimulation at non-permissive temperatures, the presynaptic terminal can be completely depleted of SVs (Estes et al., 1996). Using this mutant a total number of SVs was measured by repetitively stimulating the presynaptic nerve at non-permissive temperatures to a complete exhaustion while continuously recording synaptic currents (Delgado et al., 2000, Fig. 6). In wild-type, the amplitude of synaptic currents quickly declined first but remained at a reduced level as long as the stimulation continued (Fig. 6A left trace marked CS, expanded traces are shown in B). This is due to recycling of SVs."
||P.558 left column bottom paragraph: "On the other hand, in shibire at non-permissive temperatures, the amplitude continued to decline and finally no synaptic currents were evoked (Fig. 6A right traces, expanded traces are shown in B (traces in the right). After 350 s of tetanic stimulation no synaptic currents were observed (bottom trace with 5-fold higher amplification than other traces.) [Investigators] can estimate how many quanta are in each synaptic current (i.e., quantal content) and calculate the total number of quanta released before complete exhaustion. A total number of SVs thus determined was ∼84,000 at an abdominal longitudinal muscle, 6, in 3rd instar larvae."