Two-dimensional profiles of cells

Range Table - link
Organism Mycobacterium tuberculosis
Reference Yamada H, Yamaguchi M, Chikamatsu K, Aono A, Mitarai S. Structome analysis of virulent Mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. PLoS One. 2015 Jan 28 10(1):e0117109. doi: 10.1371/journal.pone.0117109. p.6/14 table 2PubMed ID25629354
Method p.3 bottom paragraph:"Images obtained from negative scanning through Adobe Photoshop Elements (version 9) with CanoScan 8800F were saved as TIFF files and analyzed using ImageJ and Fiji software [refs 15, 16]. Briefly, cell length was calculated by multiplying the number by 55 nm (representing the thickness of each section). The diameter (minor and major axes), perimeter, and thickness of the plasma membrane (PM), outer membrane (OM), and cell envelope of each cell were measured as a pixel value using the line selection menu in the ImageJ/Fiji window as well as a scale bar recorded on the same negative. Measured pixel values were converted to μm or nm according to the measured pixel value of the scale bar on the corresponding negatives."
Comments p.6 bottom paragraph:"Two-dimensional profiles for the five M. tuberculosis cells were calculated from values measured using ImageJ/Fiji software, as described in the Materials and Methods section. Table 2 lists the average cross-sectional area of each whole cell (outlined by the OM), its cytoplasm (outlined by the PM), and the surface areas of the OM and PM. The average cross-sectional and cytoplasmic areas were 0.116 ± 0.028 μm^2 (range, 0.079–0.147 μm^2) and 0.090 ± 0.026 μm^2 (range, 0.057–0.116 μm^2), respectively (Fig. 2), and the average OM and PM surface areas were 3.037 ± 1.333 μm^2 (range, 1.201–4.328 μm^2) and 2.672 ± 1.1911 μm^2 (range, 1.095–3.775 μm^2), respectively. Because the surface areas of cells 3, 4, and 5 were 3-fold greater than that of the smallest cell (cell 1), it is suggested that cell division may occur independently of the surface area of the parent cell."
Entered by Uri M
ID 111987