Yield characteristics of E. coli B growing in glucose-limited aerobic continuous culture over the temperature range 17.5 to 42.0˚C

Range Table - link
Organism Bacteria Escherichia coli
Reference Mainzer SE, Hempfling WP. Effects of growth temperature on yield and maintenance during glucose-limited continuous culture of Escherichia coli. J Bacteriol. 1976 Apr126(1):251-6. p.253 table 3PubMed ID770423
Method p.251 right column 2nd paragraph:"E. coli B was maintained as described previously (ref 7). The composition of the growth medium and descriptions of the continuous culture apparatus and the techniques employed in continuous culture were described previously (ref 8). Methods for the estimation of oxygen consumption, glucose utilization, acetate production, and bacterial dry weight were also given previously (ref 8). When it was necessary to carry out continuous culture at temperatures below the ambient value in the laboratory, the entire chemostat apparatus was placed in a constant-temperature room maintained at 12 C, and the temperature of the vessel was then adjusted and regulated as described previously (ref 8)."
Comments p.252 right column bottom paragraph:"Since maximal aerobic growth rates attainable at low temperatures were higher than with anaerobically grown cells, it was possible to measure yield coefficients at 17.5 C. Marked fragility of cells at temperatures above 37 C observed during anaerobic growth was not so severe when oxygen was present hence, reproducible yield values were obtained at 42 C. As before, these data can be linearly transformed to calculate the values of Yo(max) and mo (Table 3). Those values are summarized (Table 4) and plotted as a function of temperature in Fig. 1." D=growth rate
Entered by Uri M
ID 111764