Turnover of single RNase E

Value 11 cleavage events/RNase E/minute
Organism Bacteria Escherichia coli
Reference Mackie GA RNase E: at the interface of bacterial RNA processing and decay. Nat Rev Microbiol. 2013 Jan11(1):45-57. doi: 10.1038/nrmicro2930. p.51 box 2PubMed ID23241849
Primary Source Garrey, S. M. Blech, M. et al. Substrate binding and active site residues in RNases E and G: role of the 5?-sensor. J. Biol. Chem. 284, 31843–31850 (2009). doi: 10.1074/jbc.M109.063263.PubMed ID19778900
Method Primary source abstract:"To assay the relative importance of the principal RNA binding sites identified by crystallographic analysis, [investigators] introduced mutations into the 5'-sensor, the S1 domain, and the Mg(+2)/Mn(+2) binding sites."
Comments "To a first approximation, RNase E must make 150,000 cleavages in rRNA precursors, 248,000 cleavages in tRNA precursors and up to 90,000 cleavages in mRNAs (assuming three per mRNA) during every generation [BNID 111530]. This workload, 16,000 cleavage events per minute [BNID 111531], is fully consistent with an estimate of about 1,470 copies of RNase E per cell (32% of the RNA polymerase content [BNID 111532]) and a turnover of 11 cleavage events per RNase E per minute (primary source, determined with an artificial substrate at 30 °C)."
Entered by Uri M
ID 111533