Increase in cells’ angular velocity by illumination

Value 45 % Range: ±25 %
Organism Bacteria Escherichia coli
Reference Walter JM, Greenfield D, Bustamante C, Liphardt J. Light-powering Escherichia coli with proteorhodopsin. Proc Natl Acad Sci U S A. 2007 Feb 13 104(7):2408-12. p.2409 right column 2nd paragraphPubMed ID17277079
Method "To test the possibility that light-driven proton pumping is most beneficial to aerobically grown cells when their ability to respire is suddenly impaired, [researchers] energy-depleted the cells. Because E. coli is difficult to energy-deplete by nutrient limitation because of its endogenous energy stores (20, 21), [they] additionally used the respiratory poison azide, which has multiple cellular effects (22, 23) but primarily inhibits cytochrome oxidase and, thus, proton extrusion by the respiratory chain, stopping the flagellar motor (18)."
Comments "Just as [researchers] had seen upon addition of azide, PR+ cells became light-responsive upon oxygen depletion. Illumination increased the cells’ angular velocity by 45±25% (Fig. 2c P<0.005, one-sided t test)."
Entered by Uri M
ID 111063