Total amount of RNA per cell

Range by hot phenol method 1.32±0.03: by NaOH/PCA method 1.43±0.01 pg/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Miura F, Kawaguchi N, Yoshida M, Uematsu C, Kito K, Sakaki Y, Ito T. Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs. BMC Genomics. 2008 Nov 29 9: 574. p.3 table IPubMed ID19040753
Method Total amount of cellular RNAs (pg per cell) was determined for three independent preparations of S288C cells grown in YPD medium [(1%(w/v) yeast extract/2%(w/v) Bacto peptone/ 2%(w/v) glucose)] using a modified hot-phenol method [PMID 8643554] and NaOH/PCA[perchloric acid] method [PMID 1097403].
Entered by Uri M
ID 111031