Concentration of free RNAPs

Range at 0.6 doublings/hour 0.47μM: at 2.5 doublings/hour 1.1μM μM
Organism Bacteria Escherichia coli
Reference Klumpp S, Hwa T. Growth-rate-dependent partitioning of RNA polymerases in bacteria. Proc Natl Acad Sci U S A. 2008 Dec 23 105(51):20245-50. doi: 10.1073/pnas.0804953105. p.20247 right column bottom paragraphPubMed ID19073937
Comments "As shown by the red curve in Fig. 2B, the concentration of free RNAPs is predicted to increase from 0.47µM for a growth rate of 0.6 doublings per hour to 1.1µM for 2.5 doublings per hour. The range of the free RNAP concentration is substantially higher than the estimate of 30 nM made by McClure (for a doubling time of 50–60 min) based on a comparison of in vivo transcription rates from various promoters with their Michaelis constants measured in vitro (18)."
Entered by Uri M
ID 110066