Functional lifetimes of mRNA during exponential growth in LB medium and in the presence of rifampicin

Range Table - link minutes
Organism Bacteria Escherichia coli
Reference Liang ST, Ehrenberg M, Dennis P, Bremer H. Decay of rplN and lacZ mRNA in Escherichia coli. J Mol Biol. 1999 May 14 288(4):521-38. p.533 table 2PubMed ID10329160
Method "The decay of a given mRNA may be probed in at least three ways: (1) through correlations between steady state amounts and synthesis rates (2) through the enzyme induction kinetics in the case of inducible enzymes or (3) through the disappearance of its hybridization signal after a stop of transcript initiation by rifampicin. Here [researchers] have employed all three methods. This has allowed [them] to analyze the influence of rifampicin on the functional and chemical decay of lacZ and rplN mRNA sequences from the wild-type lac and spc operons, respectively, and from an artificial Pspc-rplN- lacZ fusion operon."
Comments See notes beneath table
Entered by Uri M
ID 110060