Half-lives of mRNAs encoding several ribosomal proteins (at 37°C)

Range 40 seconds - 2.5 minutes
Organism Bacteria Escherichia coli
Reference Liang ST, Ehrenberg M, Dennis P, Bremer H. Decay of rplN and lacZ mRNA in Escherichia coli. J Mol Biol. 1999 May 14 288(4):521-38. p.522 left column 2nd paragraphPubMed ID10329160
Primary Source Pedersen, S., Reeh, S. & Friesen, D. J. (1978). Functional mRNA half lives in E. coli. Mol. Gen. Genet. 166, 329-336. & Chow, J. & Dennis, P. (1994). Coupling between mRNA synthesis and mRNA stability in Escherichia coli. Mol. Microbiol. 11, 919-931.PubMed ID368581, 7517486
Method "To follow the decay of an mRNA species, transcript initiation is generally stopped with the antibiotic rifampicin (Mosteller & Yanofsky, 1970). In this manner, mRNAs encoding several ribosomal proteins from E. coli have previously been shown to decay (at 37°C) with half-lives ranging from 40 seconds to 2.5 minutes (primary sources)."
Comments "For these data to be relevant for normal growth one must assume that rifampicin does not alter the kinetics of mRNA decay."
Entered by Uri M
ID 110057