| Range |
40 seconds - 2.5 minutes
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Liang ST, Ehrenberg M, Dennis P, Bremer H. Decay of rplN and lacZ mRNA in Escherichia coli. J Mol Biol. 1999 May 14 288(4):521-38. p.522 left column 2nd paragraphPubMed ID10329160
|
| Primary Source |
Pedersen, S., Reeh, S. & Friesen, D. J. (1978). Functional mRNA half lives in E. coli. Mol. Gen. Genet. 166, 329-336. & Chow, J. & Dennis, P. (1994). Coupling between mRNA synthesis and mRNA stability in Escherichia coli. Mol. Microbiol. 11, 919-931.PubMed ID368581, 7517486
|
| Method |
"To follow the decay of an mRNA species, transcript
initiation is generally stopped with the antibiotic
rifampicin (Mosteller & Yanofsky, 1970). In
this manner, mRNAs encoding several ribosomal
proteins from E. coli have previously been shown
to decay (at 37°C) with half-lives ranging from 40
seconds to 2.5 minutes (primary sources)." |
| Comments |
"For these data to be relevant
for normal growth one must assume that
rifampicin does not alter the kinetics of mRNA
decay." |
| Entered by |
Uri M |
| ID |
110057 |