Concentration of free RNAPs (depending on growth conditions)

Range 0.5 - 1.1 μM
Organism Bacteria Escherichia coli
Reference Klumpp S. A superresolution census of RNA polymerase. Biophys J. 2013 Dec 17 105(12):2613-4. doi: 10.1016/j.bpj.2013.11.018. p.2613 right column top paragraphPubMed ID24359730
Primary Source Klumpp, S., and T. Hwa. 2008. Growth-ratedependent partitioning of RNA polymerases in bacteria. Proc. Natl. Acad. Sci. USA. 105: 20245–20250.PubMed ID19073937
Comments "The free fraction is 12%, which corresponds to ~700 RNAPs or a concentration of free RNAPs of ~1µM, much higher than an old estimate (30 nM) that has been widely used in models of gene regulation (15), but similar to a more recent estimate (0.5-1.1µM, depending on growth conditions (primary source))."
Entered by Uri M
ID 110046