||Bacteria Escherichia coli
||Wilks JC, Slonczewski JL. pH of the cytoplasm and periplasm of Escherichia coli: rapid measurement by green fluorescent protein fluorimetry. J Bacteriol. 2007 Aug189(15):5601-7 p.5606 right column 1st paragraph and fig.8BPubMed ID17545292
||In order to measure the pH of E. coli, researchers devised a procedure
based on fluorimetry of plasmid-expressed GFP mutant
3* (GFPmut3*) and YFP gene fusions.
||After HCl addition,
the pHs observed both for cytoplasm (Fig. 8A) and periplasm
(Fig. 8B) fell to just below pH 6.0. The cultures with periplasmic
GFPmut3*, showed minimal recovery of the pH (Fig.