Number of Pro-opiomelanocortin mRNA transcripts in peripheral blood mononuclear cells (PBMC)

Value 0.005 Copies/cell
Organism Human Homo sapiens
Reference van Woudenberg AD, Metzelaar MJ, van der Kleij AA, de Wied D, Burbach JP, Wiegant VM. Analysis of proopiomelanocortin (POMC) messenger ribonucleic acid and POMC-derived peptides in human peripheral blood mononuclear cells: no evidence for a lymphocyte-derived POMC system. Endocrinology. 1993 Nov133(5):1922-33PubMed ID8404638
Method Large scale preparations containing over 10(9) cells (fresh, cultured with and without Con-A) originating from several donors were examined for the presence of Pro-opiomelanocortin transcripts by analysis of poly(A)+ RNA on Northern blots and for the presence of alpha E, beta E, and gamma E by gel filtration over Sephadex G-75 and reverse phase HPLC, followed by assay of the fractions in four endorphin RIA systems with different specificities. On the Northern blots of total RNA, no POMC transcripts were detectable. In poly(A)+ RNA preparations, no full-length POMC mRNA was found, and it was estimated that the concentration of POMC mRNA, if present, was below approximately 0.005 transcript/cell in Con-A-stimulated cells and still lower in unstimulated cells.
Comments Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241 amino acid residues.
Entered by Uri M
ID 104328