Iron content in stage VI oocyte

Value 31.1 ng Range: ±3 ng
Organism African clawed frog Xenopus laevis
Reference Nomizu T, Falchuk KH, Vallee BL. Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos. Mol Reprod Dev. 1993 Dec36(4):419-23.PubMed ID8305203
Method Oocytes at different stages of maturation were taken from 6-7-cm-long female frogs anesthetized by immersion in 0.1% p-aminobenzoic acid ethyl ester, 0.3% KHCO3, pH 7.5. The abdominal cavity was opened using sterile conditions to remove a single ovarian lobe. The incision was sutured, and the animal was returned to its tank once the effects of the anesthetic ceased. then in vitro fertilization and Atomic Absorption Spectrometry.
Comments The corresponding concentration is 0.5 mM Iron in 1 microliter eggs
Entered by Uri M
ID 104005