Constant for accomodation step of tRNA selection for near cognate tRNA

Value 0.1 sec^-1
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
Primary Source Pape T, Wintermeyer W, Rodnina M. Induced fit in initial selection and proofreading of aminoacyl-tRNA on the ribosome. EMBO J. 1999 Jul 118(13):3800-7.PubMed ID10393195
Method pre-steady-state kinetic experiments.
Comments Cognate=tRNA with correct codon. Near cognate=tRNA with single nucleotide mismatch in codon.Under conditions where GTPase activation (previous step in tRNA selection) does not limit accommodation
Entered by Uri M
ID 103477