GSH [glutathione] half-lives in cells grown under different conditions

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Baudouin-Cornu P, Lagniel G, Kumar C, Huang ME, Labarre J. Glutathione degradation is a key determinant of glutathione homeostasis. J Biol Chem. 2012 Feb 10 287(7):4552-61. doi: 10.1074/jbc.M111.315705 p.4556 table 1PubMed ID22170048
Method Abstract: "Combining mathematical models and (35)S labeling, [investigators] analyzed Saccharomyces cerevisiae sulfur metabolism. This led [them] to the observation that GSH [glutathione] recycling is markedly faster than previously estimated. [They] set up additional in vivo assays and concluded that under standard conditions, GSH half-life is around 90 min."
Comments P.4555 right column bottom paragraph: "The real degradation rate of GSH is faster than the rate measured in [investigators'] assay, as some of the [35S]Cys is converted back into [35S]GSH (Fig. 1). [They] evaluated the importance of Cys back-conversion into GSH using [35S]Cys labeling (Table 1). The [Prot]Cys/[Meta]Cys ratio thus measured gives an estimate of the relative importance of the fluxes coming from Cys to proteins and to other sulfur-containing metabolites. Using these additional data in small mathematical models, [they] calculated f[GSH], defined as the fraction of GSH converted into Cys in 1 min (Table 1, supplemental information, Part 4)."
Entered by Uri M
ID 113868