Ligand [murine EGF, Epidermal Growth Factor] clearance when saturating concentrations of EGF were perfused through a liver at 35˚C in hepatocyte

Range in rapid primary phase 20,000: in slower secondary phase 2,500 molecules/min/cell
Organism Rat Rattus norvegicus
Reference Dunn WA, Hubbard AL. Receptor-mediated endocytosis of epidermal growth factor by hepatocytes in the perfused rat liver: ligand and receptor dynamics. J Cell Biol. 1984 Jun98(6):2148-59. abstractPubMed ID6327725
Method Abstract: "[Investigators] have used biochemical and morphological techniques to demonstrate that hepatocytes in the perfused liver bind, internalize, and degrade substantial amounts of murine epidermal growth factor (EGF) via a receptor-mediated process...Cytochemical localization using EGF coupled to horseradish peroxidase (EGF-HRP) revealed that the receptors were distributed along the entire sinusoidal and lateral surfaces of hepatocytes."
Comments Abstract: "When saturating concentrations of EGF were perfused through a liver at 35 degrees C, ligand clearance was biphasic with a rapid primary phase of 20,000 molecules/min per cell that dramatically changed at 15-20 min to a slower secondary phase of 2,500 molecules/min per cell. During the primary phase of uptake, approximately 250,000 molecules of EGF and 80% of the total functional receptors were internalized into endocytic vesicles which could be separated from enzyme markers for plasma membranes and lysosomes on sucrose gradients."
Entered by Uri M
ID 112726