Turgor pressure

Range E. coli ~0.3 - 3.0atm: Gram-positive bacteria such as B. subtilis and Staphylococcus aureus ∼20atm atm
Organism bacteria
Reference Mika JT, Schavemaker PE, Krasnikov V, Poolman B. Impact of osmotic stress on protein diffusion in Lactococcus lactis. Mol Microbiol. 2014 Nov94(4):857-70. doi: 10.1111/mmi.12800. p.858 left column top paragraphPubMed ID25244659
Primary Source Cayley, D.S., Guttman, H.J., and Record, M.T. (2000) Biophysical characterization of changes in amounts and activity of Escherichia coli cell and compartment water and turgor pressure in response to osmotic stress. Biophys J 78: 1748–1764. AND Whatmore, A.M., and Reed, R.H. (1990) Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation. J Gen Microbiol 136: 2521–2526. AND Deng, Y., Sun, M., and Shaevitz, J.W. (2011) Direct measurement of cell wall stress stiffening and turgor pressure in live bacterial cells. Phys Rev Lett 107: 158101.PubMed ID10733957, 2127801, 22107320
Comments P.858 left column top paragraph:"Although the amount of quantitative data is limited, it is generally thought that the turgor of E. coli (∼ 3 atm) (primary source Cayley et al., 2000) is at least an order of magnitude lower than that of Gram-positive bacteria such as B. subtilis and Staphylococcus aureus (∼ 20 atm) (primary source Whatmore and Reed, 1990). More recent measurements indicate a turgor pressure for E. coli as low as ∼ 0.3 atm (primary source Deng et al., 2011). The higher turgor of Gram-positive bacteria must reflect a higher osmolyte concentration (e.g. K+ and counter ions) and consequently it will take a larger osmotic upshift to plasmolyse Gram-positive bacteria than Gram-negative cells. Depending on the elasticity of the cell wall, the impact of osmotic stress on crowding and protein diffusion could also be different in these organisms."
Entered by Uri M
ID 112268