GSH:GSSG molar ratio

Range 50,000:1 GSH:GSSG
Organism Budding yeast Saccharomyces cerevisiae
Reference Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP. Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat Chem Biol. 2013 Feb9(2):119-25. doi: 10.1038/nchembio.1142. p.119 right column bottom paragraph & p.120 left column top paragraphPubMed ID23242256
Method "To determine the cytosolic EGSH, [investigators] introduced a Grx1-roGFP2 probe [ref 8] into wild-type BY4742 yeast cells. [They] confirmed cytosolic localization by fluorescence microscopy (Supplementary Results, Supplementary Fig. 1a). Consistent with previous observations [refs 9, 10], the cytosolic Grx1-roGFP2 probe was ~5% oxidized at steady state (Fig. 1a), which, using the published midpoint potential of roGFP2 of -280 mV [ref 7], indicates a cytosolic EGSH of -320 mV. Using an estimated total cytosolic glutathione concentration of 10 mM as a basis [ref 6], this equates to a cytosolic GSSG concentration of only 200 nM (Fig. 1a) and a GSH:GSSG molar ratio of 50,000:1."
Comments "In this study, [investigators] investigated the regulation and interplay of cytosolic glutathione homeostasis and subcellular GSSG distribution. In line with previous findings, [they] show that the cytosolic EGSH is highly reduced (~-320 mV)."
Entered by Uri M
ID 111457