Doubling times in different media

Range In glycerol 66: LB medium 22.5 minutes
Organism Bacteria Escherichia coli
Reference Liang ST, Ehrenberg M, Dennis P, Bremer H. Decay of rplN and lacZ mRNA in Escherichia coli. J Mol Biol. 1999 May 14 288(4):521-38. p.524 right column bottom paragraphPubMed ID10329160
Method "The growth medium was either Medium C (Helmstetter, 1967), supplemented with 0.2% (v/v) of glycerol and the required amino acids at 50 mg/ml, or LB medium (Miller, 1972) supplemented with 0.2% (w/v) of glucose. Experimental cultures were grown in a shaker waterbath at 37°C after inoculation from overnight cultures in glycerol minimal medium, either 1:250 into the same medium or 1:2000 into LB medium. Growth was followed by measuring the optical density at 600 nm wavelength and 1 cm light path (A600)."
Entered by Uri M
ID 110058