Average turnover rate of protein in HeLa cell

Range ~20 hours
Organism Human Homo sapiens
Reference Boisvert FM, Ahmad Y, Gierlinski M, Charrière F, Lamont D, Scott M, Barton G, Lamond AI. A quantitative spatial proteomics analysis of proteome turnover in human cells. Mol Cell Proteomics. 2012 Mar11(3):M111.011429. doi: 10.1074/mcp.M111.011429. abstract & p.6 right column 3rd paragraphPubMed ID21937730
Method "[Researchers] combine an enhanced pulse SILAC (stable isotope labeling with amino acids in cell culture) approach with spatial proteomics to perform a system-wide analysis of protein turnover in cultured human cells."
Comments "Approximately 60% of HeLa proteins have 50% turnover values clustered within 5 h of the average turnover rate of 20 h (Fig. 3, blue lines). However, if [researchers] correct for protein abundance, it takes 24 h for 50% turnover of the total HeLa proteome, because a subset of abundant proteins has turnover values longer than the mean of 20 h."
Entered by Uri M
ID 109937