Steady-state and microscopic kinetic parameters

Range Table - link
Organism Bacteria Escherichia coli
Reference Uter NT, Perona JJ. Long-range intramolecular signaling in a tRNA synthetase complex revealed by pre-steady-state kinetics. Proc Natl Acad Sci U S A. 2004 Oct 5 101(40):14396-401. p.14398 table 1PubMed ID15452355
Method "A highly sensitive and generalizable assay for tRNA aminoacylation was adapted for rigorous measurement of steady-state and microscopic rate constants by E. coli GlnRS. The assay is based on 32P-labeling at the 3'-internucleotide linkage of the tRNA (ref 18)."
Entered by Uri M
ID 109638