Average number density of RNAP within the cytoplasm

Value 2200 RNAP/μm^3 Range: 1,800 to 3,500 RNAP/μm^3
Organism Bacteria Escherichia coli
Reference Bakshi S, Siryaporn A, Goulian M, Weisshaar JC. Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells. Mol Microbiol. 2012 Jul85(1):21-38. doi: 10.1111/j.1365-2958.2012.08081.x. p.27 right column 2nd paragraphPubMed ID22624875
Method Superresolution fluorescence microscopy
Comments "...[researchers] use the total RNAP–yGFP intensity and the known geometry to calculate the average number density of RNAP within the cytoplasm. This varies across cells from 1800 to 3500 RNAP per cubic micrometre, with a mean of 2200 RNAP per cubic micrometre."
Entered by Uri M
ID 108605