Number of pom1p per cell

Value 5000 Molecules/cell Range: ±1900 Molecules/cell
Organism Fission yeast Schizosaccharomyces pombe
Reference Saunders TE, Pan KZ, Angel A, Guan Y, Shah JV, Howard M, Chang F. Noise reduction in the intracellular pom1p gradient by a dynamic clustering mechanism. Dev Cell. 2012 Mar 13 22(3):558-72. p.559 right column 2nd paragraphPubMed ID22342545
Method "...comparing the fluorescence intensity of pom1-GFP with other GFP fusions as standards"
Comments "By comparing the fluorescence intensity of pom1-GFP with other GFP fusions as standards (see Figure S1B) (Wu and Pollard, 2005), [researchers] estimated that there are approximately 5,000 ± 1,900 molecules of pom1p per cell, with approximately 2,500 molecules located at the cell tips (a tip region is defined here as the 10% of the cell closest to a cell tip)."
Entered by Uri M
ID 108377