PPi concentration in cell

Range 0.9 to 1.3 mM
Organism Bacteria Escherichia coli
Reference Chen J, Brevet A, Fromant M, Lévêque F, Schmitter JM, Blanquet S, Plateau P. Pyrophosphatase is essential for growth of Escherichia coli. J Bacteriol. 1990 Oct172(10):5686-9. p.5687 right column 3rd paragraphPubMed ID2170325
Method Pyrophosphatase activity in the cell extract was measured in a 0.1-ml assay mixture containing 25 mM Tris hydrochloride (pH 7.8), 20 mM KCl, 2.5 mM MgCl2, 0.1 mM PPi, 100,000 dpm of 32PP, and appropriately diluted crude extract solution.
Comments During growth, cellular PPi remained nearly constant, between 0.9 and 1.3 mM. It is noteworthy that this concentration is identical to that in the parental strain K37.
Entered by Uri M
ID 108173