Diffusion coefficients of very large, 3200 kDa, membrane-embedded flagellar basal bodies

Range ~0.005 μm^2/sec
Organism Bacteria Escherichia coli
Reference Kumar M, Mommer MS, Sourjik V. Mobility of cytoplasmic, membrane, and DNA-binding proteins in Escherichia coli. Biophys J. 2010 Feb 17 98(4):552-9. p.558 right column top paragraphPubMed ID20159151
Primary Source Fukuoka H, Sowa Y, Kojima S, Ishijima A, Homma M. Visualization of functional rotor proteins of the bacterial flagellar motor in the cell membrane. J Mol Biol. 2007 Mar 30 367(3):692-701PubMed ID17289075
Method Researchers used fluorescence recovery after photobleaching (FRAP) along with a detailed numerical model that simulates FRAP experiments, to systematically investigate mobility of cytoplasmic, inner membrane, and DNA-binding proteins in Escherichia coli.
Comments Very large, 3200 kDa, membrane-embedded flagellar basal bodies, with several tens of transmembrane helices, move with apparent diffusion coefficients of ~0.005µm^2/sec (primary source).
Entered by Uri M
ID 107987