Quantitation of ppGpp for three cultures of E. coli B/r grown at 30°C

Range Table - link
Organism Bacteria Escherichia coli
Reference Ryals J, Little R, Bremer H. Temperature dependence of RNA synthesis parameters in Escherichia coli. J Bacteriol. 1982 Aug151(2):879-87. p.883 table 6PubMed ID6178724
Method At an OD460 of approximately 1.0, 120 ml of culture that had grown exponentially for at least 10 generations was treated with 12 ml of 1.8% formaldehyde and cooled on ice for 20 min. See notes beneath table
Entered by Uri M
ID 107929