Kinetic parameters for the hydrolysis of chlorpyrifos by organophosphorous hydrolase wildtype and variants

Range Table - link
Organism Bacteria Escherichia coli
Reference Cho CM, Mulchandani A, Chen W. Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos. Appl Environ Microbiol. 2004 Aug70(8):4681-5.PubMed ID15294802
Method Directed evolution. Researchers used two rounds of DNA shuffling and screened about 12,000 mutants to improve the hydrolytic activity of an organophosphorus hydrolase (OPH) 725-fold toward the toxin chlorpyrifos.
Entered by Uri M
ID 105432