Cell diameter and length (average±standard deviation) obtained from phase contrast microscopy (PCM)

Range Diameter 0.85±0.07: Length 2.85±0.66 μm
Organism Bacteria Escherichia coli
Reference Hiroyuki Yamada et al., Structome analysis of Escherichia coli cells by serial ultrathin sectioning reveals the precise cell profiles and the ribosome density, Microscopy, Volume 66, Issue 4, August 2017, Pages 283–294, link p.287 table 1 PubMed ID28854579
Method P.286 left column bottom paragraph: "In this study, E. coli was selected as a target of the structome analysis because a large number of molecular biological studies were performed with this microorganism. E. coli cells were cut into 65 nm thick serial ultrathin sections after rapid cryofixation and freeze-substitution. In addition to the structome analysis by serial ultrathin sectioning, PCM (phase contrast microscopy) and SEM (scanning electron microscopy) examinations were performed on the same bacterial sample because the fundamental morphological studies were achieved with these observation techniques."
Comments P.286 right column, bottom paragraph: "In addition to the structome analysis on the serial ultrathin sections of nine E. coli cells, PCM and SEM examinations were performed in order to determine one-dimensional measurements on 131 and 44 E. coli cells, respectively. Data obtained from three analysis methods were compared. Table.1 shows the results of one-dimensional analysis examined by PCM, SEM and serial ultrathin sections with transmission electron microscopy (TEM)." P.287 left column bottom paragraph: "However, in the result obtained from SEM measurement, average cell diameter was 0.49 μm with the smallest SD of 0.03 and was significantly smaller than those of PCM and structome analysis based on examination of serial ultrathin sections (both P < 0.001). Average diameter of cytoplasm at PM was 0.85 ± 0.06 μm measured with only structome analysis based on serial ultrathin sections because PM cannot be visualized with SEM and PCM analysis (Table 1)."
Entered by Ron Milo - Admin
ID 117345