Method |
P.2 right column 2nd paragraph: "To assess the role of Cx43 in directional cell migration, [investigators] generated primary mouse embryonic fibroblasts (MEFs) from Cx43 wildtype and KO [knock out] mouse embryos for assessment of cell motility using a well described wound healing assay [refs 33 ,48, 49]. Briefly, this entailed growing the MEFs to confluence followed by 48 hrs of serum starvation. Then a scratch is introduced across the monolayer to generate a small wound or gap, and serum is restored which stimulates cell migration across the gap to close the wound. Typically with wildtype cells, the gap is quickly filled in a few hours. However, with Cx43KO MEFs, wound closure occurred more slowly (Fig 1)." |