Average migration rate of primary mouse embryonic fibroblasts (MEFs) at the advancing wound edge

Value 20.7 µm/hour Range: ±1.5 µm/hour
Organism Mouse Mus musculus
Reference Francis R et al., Connexin43 modulates cell polarity and directional cell migration by regulating microtubule dynamics. PLoS One. 2011 6(10):e26379. doi: 10.1371/journal.pone.0026379 p.2 right column 2nd paragraphPubMed ID22022608
Method P.2 right column 2nd paragraph: "To assess the role of Cx43 in directional cell migration, [investigators] generated primary mouse embryonic fibroblasts (MEFs) from Cx43 wildtype and KO [knock out] mouse embryos for assessment of cell motility using a well described wound healing assay [refs 33 ,48, 49]. Briefly, this entailed growing the MEFs to confluence followed by 48 hrs of serum starvation. Then a scratch is introduced across the monolayer to generate a small wound or gap, and serum is restored which stimulates cell migration across the gap to close the wound. Typically with wildtype cells, the gap is quickly filled in a few hours. However, with Cx43KO MEFs, wound closure occurred more slowly (Fig 1)."
Comments P.2 right column 2nd paragraph: "Quantitation of the rate of wound closure by measuring the advancing wound edge showed wildtype MEFs migrated at an average rate of 20.7±1.5 µm/hour, as compared to 8.1±1.3 µm/hour for the Cx43 KO MEFs (Fig. 1E)."
Entered by Uri M
ID 117159