| Range |
at the cytoplasmic side of the channel ~40: near the extracellular membrane surface 14: in the extracellular space 25 Å
|
| Organism |
Human Homo sapiens |
| Reference |
Maeda S et al., Structure of the connexin 26 gap junction channel at 3.5 A resolution. Nature. 2009 Apr 2458(7238):597-602. doi: 10.1038/nature07869 p.598 left column top paragraphPubMed ID19340074
|
| Method |
Abstract: "Here [investigators] report the crystal structure of the gap junction channel formed by human connexin 26 (Cx26, also known as GJB2) at 3.5 A resolution, and discuss structural determinants of solute transport through the channel." P.601 right column bottom paragraph: "Methods summary: Human Cx26 was expressed in Sf9 insect cells using recombinant baculovirus. The gap junction channel was solubilized in dodecylmaltoside and purified sequentially by cation exchange and size-exclusion chromatography. Crystals were grown by the hanging-drop vapour diffusion method with PEG200 as a precipitant. The structure was determined by the single-isomorphous replacement combined with anomalous scattering (SIRAS) method, with phase extension by six-fold non-crystallographic (NCS) averaging." |
| Comments |
P.598 left column top paragraph: "The diameter of the pore is about 40 Å at the cytoplasmic side of the channel, narrowing to 14 Å near the extracellular membrane surface and then widening to 25 Å in the extracellular space." |
| Entered by |
Uri M |
| ID |
117151 |