| Range |
by 15 minutes of synthesis
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Lampe PD, Lau AF. Regulation of gap junctions by phosphorylation of connexins. Arch Biochem Biophys. 2000 Dec 15 384(2):205-15 DOI: 10.1006/abbi.2000.2131 p.208 right column bottom paragraphPubMed ID11368307
|
| Primary Source |
[19] Crow DS, Beyer EC, Paul DL, Kobe SS, Lau AF. Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts. Mol Cell Biol. 1990 Apr10(4):1754-63 DOI: 10.1128/mcb.10.4.1754PubMed ID1690850
|
| Method |
Primary source [19] abstract: "Uninfected fibroblast 45- and 47-kDa proteins were phosphorylated on serine residues. Phosphatase digestions of 45- and 47-kDa proteins and pulse-chase labeling studies indicated that these proteins represented phosphorylated forms of the 43-kDa protein." |
| Comments |
P.208 right column bottom paragraph: "The initial phosphorylation of Cx43 can occur by 15 min of synthesis in cultured fibroblast cells (primary source)." Primary source p.1759 right column bottom paragraph: "Pulse-chase labeling studies of connexin43: To estimate the rapidity of serine site phosphorylation in connexin43-related proteins, uninfected vole fibroblasts were pulse-labeled with [35S]methionine for 15 min and then chased for various times in medium containing nonradioactive methionine." |
| Entered by |
Uri M |
| ID |
117149 |