Number of copies of prestin mRNA that can be found per OHC (outer hair cell) by postnatal day 3 (P3)

Range ~300 prestin copies/OHC
Organism Rat Rattus norvegicus
Reference Ashmore J. Cochlear outer hair cell motility. Physiol Rev. 2008 Jan88(1):173-210. doi: 10.1152/physrev.00044.2006 p.193 right column 3rd paragraphPubMed ID18195086
Primary Source [123] Gross J, Machulik A, Amarjargal N, Fuchs J, Mazurek B. Expression of prestin mRNA in the organotypic culture of rat cochlea. Hear Res. 2005 Jun204(1-2):183-90 DOI: 10.1016/j.heares.2005.02.001PubMed ID15925203
Method Primary source abstract: "To quantitate in absolute terms the prestin mRNA levels in the explant culture of rat cochlea, [investigators] used competitive RT-PCR with a synthetic internal cRNA standard."
Comments P.193 right column 3rd paragraph: "By postnatal day 3 (P3) in the rat, ~300 copies of prestin mRNA can be found per OHC (primary source)." Primary source abstract: "Prestin gene expression was found at levels of 100 fg specific mRNA/microg total RNA on postnatal day 3, which corresponds to about 300 copies per outer hair cell (OHC) and is indicative of an intermediate level of expression."
Entered by Uri M
ID 117138