Fraction of perivascular macrophages that can be replaced within 3 months

Value 80 %
Organism Mammals
Reference Daneman R, Prat A. The blood-brain barrier. Cold Spring Harb Perspect Biol. 2015 Jan 5 7(1):a020412. doi: 10.1101/cshperspect.a020412 p.5 right column top paragraphPubMed ID25561720
Primary Source Unger ER, Sung JH, Manivel JC, Chenggis ML, Blazar BR, Krivit W. 1993. Male donor-derived cells in the brains of female sex-mismatched bone marrow transplant recipients: A Y-chromosome specific in situ hybridization study. J Neuropathol Exp Neurol 52: 460–470 DOI: 10.1097/00005072-199309000-00004 AND Vass K, Hickey WF, Schmidt RE, Lassmann H. 1993. Bone marrow-derived elements in the peripheral nervous system. An immunohistochemical and ultrastructural investigation in chimeric rats. Lab Invest 69: 275–282 AND Williams K, Alvarez X, Lackner AA. 2001. Central nervous system perivascular cells are immunoregulatory cells that connect the CNS with the peripheral immune system. Glia 36: 156–164PubMed ID8103085, 8377470, 11596124
Method Primary source Unger et al., 1993, abstract: "In five female bone marrow transplant (BMT) recipients of sex-mismatched donor marrow, Y-chromosome specific in situ hybridization was performed on formalin-fixed, paraffin-embedded sections of the medulla to detect the male donor marrow-derived cells. Y-chromosome-bearing cells (Y-cells), thereby donor-derived, were matched with leukocyte common antigen (LCA)-reactive cells in adjacent sections immunostained with anti-LCA antibody." Primary source Vass et al., 1993, abstract: "Inbred DA rats were lethally irradiated and transplanted with (Lewis x DA)F1 bone marrow carrying the non-DA major histocompatibility antigens from Lewis rats. The anti-RT-1A antibody (anti-Lewis MHC class I), I1-69, and the macrophage markers, Ed2 and Ox42 were used to visualize immunohistochemically donor bone marrow-derived cells and resident macrophages of sensory and autonomic ganglia as well as in peripheral nerves."
Comments P.5 left column bottom paragraph: "CNS blood vessels interact with different immune cell populations both within the blood as well as within the CNS. The two main cell populations within the CNS are perivascular macrophages and microglial cells. Perivascular macrophages are monocyte lineage cells that sit on the ablumenal side of the vascular tube commonly found in the Virchow–Robin space, a small fluid filled canal that lines the ablumenal surface of the veins and arteries that enter/leave the CNS (Hickey and Kimura 1988, Polfliet et al. 2001). These cells are derived from blood-borne progenitors, and chimera experiments suggest that they are able to cross the BBB [blood-brain barrier] and can be 80% replaced within 3 mo (primary sources)." Primary source Vass et al., 1993, abstract: "In the ganglia, up to 80% and within peripheral nerves, up to 60% of macrophages were replaced by donor cells within 3 months of established chimerism." Primary source Unger et al., 1993, studied humans. Primary source Vass et al., 1993, studied rats.
Entered by Uri M
ID 117041