Number of target genes directly regulated by the Twist transcription factor during embryogenesis

Range ~500 target genes
Organism Fruit fly Drosophila melanogaster
Reference Carroll SB. Evo-devo and an expanding evolutionary synthesis: a genetic theory of morphological evolution. Cell. 2008 Jul 11 134(1):25-36. doi: 10.1016/j.cell.2008.06.030 p.30 right column top paragraph and 2nd paragraphPubMed ID18614008
Primary Source Sandmann T et al., A core transcriptional network for early mesoderm development in Drosophila melanogaster. Genes Dev. 2007 Feb 15 21(4):436-49 DOI: 10.1101/gad.1509007PubMed ID17322403
Method Primary source abstract: "Here, [investigators] report the characteristics of the regulatory network orchestrating early mesodermal development in the fruitfly Drosophila, where the transcription factor Twist is both necessary and sufficient to drive development. Through the integration of chromatin immunoprecipitation followed by microarray analysis (ChIP-on-chip) experiments during discrete time periods with computational approaches, [they] identified >2000 Twist-bound cis-regulatory modules (CRMs) and almost 500 direct target genes."
Comments P.30 right column top paragraph: "Even greater is the number of target genes directly regulated by the Drosophila Twist transcription factor during embryogenesis—nearly 500 targets in total, including genes involved in cell proliferation, cell migration, and morphogenesis, as well as nearly one-fourth of all known transcription factor genes (primary source)." P.30 right column 2nd paragraph: "The scale of the Twist-regulated network, for example, means that, in the course of evolution, some 500 linkages have evolved between Twist and different CREs [cis-regulatory elements]."
Entered by Uri M
ID 117008