| Range |
erythrocytes 0 copies/cell: sperm cells ~5copies/cell: oocytes >500,000 copies/cell
|
| Organism |
Mammals |
| Reference |
Shokolenko IN, Alexeyev MF. Mitochondrial DNA: A disposable genome? Biochim Biophys Acta. 2015 Sep1852(9):1805-9. doi: 10.1016/j.bbadis.2015.05.016 p.1807 left column 2nd paragraphPubMed ID26071375
|
| Primary Source |
[19] Gabriel MS, Chan SW, Alhathal N, Chen JZ, Zini A. Influence of microsurgical varicocelectomy on human sperm mitochondrial DNA copy number: a pilot study. J Assist Reprod Genet. 2012 Aug29(8):759-64. doi: 10.1007/s10815-012-9785-z [20] Reynier P et al., Mitochondrial DNA content affects the fertilizability of human oocytes. Mol Hum Reprod. 2001 May7(5):425-9PubMed ID22562241, 11331664
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| Method |
Primary source [19] abstract: "Surgical procedure: Microsurgical sub-inguinal varicocelectomy. Outcome measurements and statistical analysis: Conventional sperm parameters, sperm mtDNA copy number (by real time PCR) and sperm chromatin structure assay (SCSA) parameters (%DFI,% HDS) before and 4 months after microsurgical varicocelectomy." Primary source [20] abstract: "In the present study, real-time quantitative polymerase chain reaction analysis of 113 unfertilized oocytes obtained from 43 patients revealed an average of 193,000 (range: 20,000 to 598,000) mitochondrial genomes per cell." |
| Comments |
P.1807 left column 2nd paragraph: "mtDNA is maintained at different copy numbers in different tissues, the two extremes of this spectrum being mammalian erythrocytes and sperm which have no mtDNA and ~ 5 copies of mtDNA per cell [primary source 19], respectively, and oocytes, which may contain > 500,000 copies [primary source 20]. In Drosophila, mtDNA is eliminated from the sperm in the male genital tract prior to fertilization, so that fertilizing sperm may contain no mtDNA at all [ref 21]. Curiously, human oocyte quality directly correlates with mtDNA copy number, whereas this correlation is inverse for the human sperm [primary source 20]." |
| Entered by |
Uri M |
| ID |
116471 |