| Comments |
P.163, bottom of page: "Abbreviations used: Con A, concanavalin A FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone JATP, flux through ATP in units of the
oxygen consumption used to drive it C, control coefficient, defined as the fractional change in a variable (usually flux) caused by an infinitesimal
fractional change in a system parameter when the system is allowed to relax to a new steady state Ɛ, elasticity, defined as the fractional change in
a local flux caused by an infinitesimal fractional change in a system variable when the system is not allowed to relax Δѱ, mitochondrial membrane potential." P.164 left column 2nd paragraph: "These data are similar to those [investigators] have described previously in this and other media, where [they] accounted for more than 80 % of cellular respiration, with proton leak contributing 20-40 % of the total [primary source, refs 8, 10]." P.165 right column 3rd paragraph: "Control over the reactions around cytoplasmic ATP: ATP producers and ATP consumers-First, [investigators] calculated the control over ATP production rate exerted by each of the individual blocks of ATP-consuming reactions (protein synthesis, sodium cycling, calcium cycling, RNA/DNA synthesis and unidentified ATP consumers). By subtracting the oxygen consumption used to drive the proton leak [primary source] from the total oxygen consumption rate, [they] calculated the rate of ATP production in terms of the oxygen consumption needed to drive it (glycolytic ATP production is negligible under [their] conditions). From the measured rates of the ATP consumers [they] then calculated the fraction of ATP production used by each of the individual blocks of ATP-consuming reactions (Table 1). From the branching theorem of control analysis [ref 21] [they] know that control over the rate of ATP production is divided among the ATP consumers in the ratio of their fluxes. The reactions with the largest fluxes had the greatest share of the control over ATP production rate (Table 1)."
See note above table |