Fraction of all intracellular water that displays translational (macroscopic) and rotational (microscopic) dynamics that are indistinguishable from pure water

Range ~85 %
Organism Generic
Reference Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6665 right column 2nd paragraphPubMed ID24901537
Primary Source [108] Jasnin M, Stadler A, Tehei M, Zaccai G. Specific cellular water dynamics observed in vivo by neutron scattering and NMR. Phys Chem Chem Phys. 2010 Sep 21 12(35):10154-60. doi: 10.1039/c0cp01048k [109] Qvist J, Persson E, Mattea C, Halle B. Time scales of water dynamics at biological interfaces: peptides, proteins and cells. Faraday Discuss. 2009 141 :131-44 discussion 175-207PubMed ID20714607, 19227355
Method Primary source [108] abstract: "Neutron scattering, by using deuterium labelling, revealed how intracellular water dynamics, measured in vivo in E. coli, human red blood cells and the extreme halophile, Haloarcula marismortui, depends on the cell type and nature of the cytoplasm. The method uniquely permits the determination of motions on the molecular length (approximately ångstrøm) and time (pico- to nanosecond) scales." Primary source [109] abstract: "Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. The dynamical heterogeneity of hydration water is characterized by performing relaxation measurements over a wide temperature range, extending deeply into the supercooled regime, or by covering a wide frequency range. Protein hydration layers can be described by a power-law distribution of rotational correlation times with an exponent close to 2."
Comments P.6665 right column 2nd paragraph: "Concerning water motions on the pico- (ps) to nano- (ns) second time scale, corresponding to the subnanometer (nm) length scale, ∼85% of all intracellular water displays translational (macroscopic) and rotational (microscopic) dynamics that are indistinguishable from pure water, both in prokaryotic and eukaryotic cells (primary sources). This amount of bulk water is not coordinated in the first hydration shell of cellular macromolecules and only marginally affects the rotational dynamic properties of small fluorescent dyes (ref 110)."
Entered by Uri M
ID 115853